Journal: Synthetic and Systems Biotechnology

Article Title: AmXlnR, a transcription factor involved in xylan degradation and pentose catabolism, enhances pullulan production from xylose in Aureobasidium melanogenum

doi: 10.1016/j.synbio.2026.02.007

Figure Lengend Snippet: In vivo G FP expression analysis control of the native and mutated promoters of AmXLN2 , AmBXL2 and AmABF1 . ( a ) Schematic representation of the native promoters and their variants with deleted putative XlnR-binding sites: P XLN2 Δ:P XLN2 with 5′-GGCTGA-3′ deleted; P BXL2 Δ:P BXL2 with 5′-GGTTAA-3′ deleted; P ABF1 Δ:P ABF1 with 5′-GGCTAT-3′ deleted. ( b ) Relative fluorescence intensity, ( c ) relative GFP transcriptional level, and ( d ) bright-field and corresponding fluorescence images of the reporter strains. Three independent replicates were performed for the statistical analysis. ∗ P < 0.05,∗∗ P < 0.01, ns: no significance.

Article Snippet: GFP fluorescence intensity was visualized using an Olympus U-LH100HG fluorescent microscope and quantified using a BioTeK Synergy H1 Hybrid Reader (BioTek Instruments Inc., USA) (485 nm excitation and 520 nm emission).

Techniques: In Vivo, Expressing, Control, Binding Assay, Fluorescence

Journal: Journal of Advanced Research

Article Title: Airway basal stem cell-derived extracellular vesicles drive ECM remodeling and suppress fibroblasts activation via the miR-30a-5p/FAP axis in benign tracheal stenosis

doi: 10.1016/j.jare.2025.08.014

Figure Lengend Snippet: Quantification, characterization, and internalization assays of BSC-EVs. (a) Representative fluorescent immunocytochemistry images showing KRT5 (green), MUC5B (green), AC-TUB (green), P63 (red), and KI67 (red) expression in BSCs, with nuclei counterstained using DAPI (blue). Scale bars, 100 μm. (b) HE staining demonstrates the formation of a pseudostratified epithelium by BSCs, comprising ciliated and mucus-producing cells under air–liquid interface differentiation. Scale bar, 30 μm. (c) Nano-flow cytometry analysis reveals the particle size distribution of BSC-EVs. (d) Representative transmission electron microscopy images of BSC-EVs. Scale bar, 300 μm. (e) Western blot analysis confirms the presence of EV markers, including TSG101, HSP70, CD81, CD9, and CD63. #1, #2, #3, #4, and #5 represent BSC-EVs from three different human samples, respectively. (f) Tracking of intratracheally injected PKH-26-labeled BSC-EVs (red) in the rabbit trachea over 14 days, with representative gross, fluorescent stereomicroscope, and fluorescence microscopy images presented sequentially.

Article Snippet: Mean fluorescence intensity was quantified using Celigo software (top right).

Techniques: Immunocytochemistry, Expressing, Staining, Flow Cytometry, Transmission Assay, Electron Microscopy, Western Blot, Injection, Labeling, Fluorescence, Microscopy

Journal: Journal of Advanced Research

Article Title: Airway basal stem cell-derived extracellular vesicles drive ECM remodeling and suppress fibroblasts activation via the miR-30a-5p/FAP axis in benign tracheal stenosis

doi: 10.1016/j.jare.2025.08.014

Figure Lengend Snippet: Assessment of fibroblasts activation and ECM remodeling of miR-30a-5p-containing BSC-EVs in an indirect co-culture system. (a) Schematic representation of the co-culture model for AFs and BSCs transfected with NC or miR-30a-5p, with either DMSO or GW4869 as treatments. (b) Representative fluorescent immunocytochemistry images showing FAP expression in AFs treated with NC inhibitors + DMSO, NC inhibitors + GW4869, miR-30a-5p inhibitors + DMSO, and miR-30a-5p inhibitors + GW4869 (n = 3 per group). Scale bar = 100 μm (top left). Mean fluorescence intensity was quantified using Celigo software (top right). Collagen contractility assays showing representative collagen gel images post-release (bottom left) were quantified for contraction area using ImageJ software (bottom right). (c) Western blot analysis of protein levels for Collagen I, FAP, α-SMA, MMP2, MMP7, MMP9, TIMP1, TIMP2, and TIMP3 in the four treatment groups. (d) Representative images of collagen gels post-release in AFs treated with NC mimics + DMSO, NC mimics + GW4869, miR-30a-5p mimics + DMSO, and miR-30a-5p mimics + GW4869. (e) Corresponding western blot analysis of Collagen I, FAP, α-SMA, MMP2, MMP7, MMP9, TIMP1, TIMP2, and TIMP3 protein levels in these four groups.

Article Snippet: Mean fluorescence intensity was quantified using Celigo software (top right).

Techniques: Activation Assay, Co-Culture Assay, Transfection, Immunocytochemistry, Expressing, Fluorescence, Software, Western Blot